Evaluation of a latex agglutination test for detection of antibodies to rubella virus in selected sera.
نویسندگان
چکیده
A new latex agglutination test for rubella virus was used to test sera with a hemagglutination inhibition titer of less than or equal to 8 (97 specimens) or greater than or equal to 256 (158 specimens). Maximum latex agglutination test sensitivity was achieved when low-titer sera were tested undiluted and high-titer sera were diluted (1:10). A modification of the protocol of the manufacturer resulted in a latex agglutination-hemagglutination inhibition agreement of 96.7%.
منابع مشابه
Evaluation of a Latex Agglutination Test for Detection of Antibodies to Rubella Virus in Selected Sera
A new latex agglutination test for rubella virus was used to test sera with a hemagglutination inhibition titer of -8 (97 specimens) or >256 (158 specimens). Maximum latex agglutination test sensitivity was achieved when low-titer sera were tested undiluted and high-titer sera were diluted (1:10). A modification of the protocol of the manufacturer resulted in a latex agglutination-hemagglutinat...
متن کاملComparison of the latex agglutination test with the hemagglutination inhibition test, enzyme-linked immunosorbent assay, and neutralization test for detection of antibodies to rubella virus.
The ability of a rapid, latex agglutination test to diagnose rubella infection and to measure immune status was evaluated by comparison with the hemagglutination-inhibition (HAI) test, enzyme-linked immunosorbent assay (ELISA), and the neutralization (NT) test. The latex agglutination test accurately detected serological conversions in 74 pairs of sera representing 21 natural infections and 53 ...
متن کاملComparison of a simple latex agglutination test with hemolysis-in-gel, hemagglutination inhibition, and radioimmunoassay for detection of rubella virus antibodies.
Rubella virus antibodies were measured in 300 sera from pregnant women visiting a maternity center by using a new, simple latex test, Rubalex. The results were compared with those obtained by using hemolysis in gel, hemagglutination inhibition, and radioimmunoassay. The sensitivity of the latex test was 100, 98.0, and 99.6% when compared with hemolysis in gel, hemagglutination inhibition, and r...
متن کاملEvaluation of Antibodies against Rubella Virus in a Mass Campaign Vaccination in Tehran, Iran
Background and Aims: Vaccine is available, but fetal infection with rubella virus is still a main cause of congenital birth defects and mental retardation in many countries. Mass vaccination campaigns and Expanded Program of Immunization (EPI) have increased vaccine coverage in the world with a substantial impact on the reduction of rubella infections, such as Congenital Rubella Syndrome (CRS)....
متن کاملLatex enzyme immunoassay for measuring IgG antibodies to rubella virus.
A latex enzyme immunoassay (LEIA) for detecting IgG class antibody to rubella virus was compared with the latex agglutination test (LA) and a haemagglutination inhibition assay (HI). Of 243 sera tested, four discrepant results were observed among all three techniques, corresponding to borderline values. Except for one sample containing specific IgM class antibody, the difference between quantit...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 18 1 شماره
صفحات -
تاریخ انتشار 1983